Labs 4a,b,i,j
the purpose of this experiment was to form solutions that would act as different substances in the body that split and form DNA.
materials:
materials:
- analytic balance
- tabletop balance
- weigh materials
- lap scoopers
- sodium chloride
- test tubes
- test tube racks
- TRIS
- EDTA
- 95% Ethanol solution
- beakers (600 ml)
- bottle (125 ml)
- graudtaed cylinder
- ph testing paper
- hydrochloric acid
- sodium hydroxide
- glass rods
- beaker (50 ml)
- DNA (extracted salmon sperm)
- pipet (2 ml)
- agarose
- microwave
- hand protector thingy
- science goggles
- gel-formed cube
- electric power supply
- Centrifuge (spinny thing)
- E-Bromide
- pipet tips
- pipet pumps
Procedure:
4A:
Fr this lab, everything went very wrong. we noticed in the end not only did the DNA not travel, but it just failed in general. It could have had something to do with our materials expiring or just straight up human error. We will most likely try this lab again,if it had succeeded we could have strengthened our knowledge in forensic procedures.
Reflection:
Our experiment unfortunately failed, i learned not much for this lab, but i learned that mistakes will be made. Since mistakes will be made, blaming someone is necessary. Just kidding, totally not though. I didn't know how to spool DNA and now,... i still don't.
4A:
- Measure 2.292 grams of NaCl
- Add the NaCl t a test tube
- Add water to the test tube
- Add until solution reaches 10 ml
- Add 0.1576 g of TRIS to a beaker
- Add 0.037224 g of EDTA to a beaker
- Add 80 ml of the deionized water to the solution in the tube
- Add hcl to increase ph or add naoh to decrease ph of solution
- Alter ph solution between 8,5 and 7,5
- Add water in solution until it reaches 100 ml
- Label so no one messes up
- Add 1 ml of "DNA" to a beaker
- Add 1 ml of TE to a beaker
- Add 5 ml of NaCl to the beaker
- Pour down 4 ml of ETH making two layers
- Spool DNA with the glass rod mentioned above
- Place all the DNA in the tube with everything in it
- Add 0,4 grams of the agarose to the solution and add TE solution to it until 100 ml is reached
- Heat up solution until it is boiling and agarose is dissolved
- Wait until solution cools
- Pour into gel container
- Pour TAE until gel box is completely submerged
- Add 20 uL DNA into a tube
- add 4mL of 6x loading dye into the tube
- Shake the solution using the centrifuge
- Put solution into gel
- Wait 45 minutes while gel is being charged
- Stain with the E-Bromide, rinse it, and observe
Fr this lab, everything went very wrong. we noticed in the end not only did the DNA not travel, but it just failed in general. It could have had something to do with our materials expiring or just straight up human error. We will most likely try this lab again,if it had succeeded we could have strengthened our knowledge in forensic procedures.
Reflection:
Our experiment unfortunately failed, i learned not much for this lab, but i learned that mistakes will be made. Since mistakes will be made, blaming someone is necessary. Just kidding, totally not though. I didn't know how to spool DNA and now,... i still don't.