The purpose of this lab is to find out what substances can be used as positive indicator tests for fats, proteins, and carbohydrates. We also have to find out if an egg or parts of an egg test positive for proteins, carbohydrates or fats?
The materials that are going to be used for this lab are:
Part 1: Separating Cell Structures
Lipid Indicator Test
In the first day of this lab, we were tasked with placing an egg in a beaker filled to the top with vinegar, to see the reaction that would occur between the egg and the vinegar.
On the second day that we observed the egg, the eggs shell was dissolved. To remove all remnants of the H20 from the egg, the egg was placed in sat water.
On the third day of observing the egg, we needed to rehydrate the egg, It was placed back into distilled water. At that point, we cut the egg open to remove all the parts of it to test them, which we did. We were meant to collect the data, which is below.
The materials that are going to be used for this lab are:
- Eggs
- Beaker, 250 ml
- White vinegar
- Plastic Wrap
- Slotted spoon
- Sodium Chloride
- Distilled Water
- Beaker, 110 ml
- Pipets, 5 ml
- Pipet pump, green
- Tubes, glass, 13x100 mm
- Peg racks for 13x100mm tubes
- Glucose (dextrose)
- Benedict's Solution
- Hot Plate stirrer
- Test tube holder
- Starch, soluble
- Lugol's Iodine Solution
- Vortex mixer
- Gelatin
- Sodium hydroxide
- Cupric sulfate 5-hydrate
- Oil
- Sudean IV Solution
- Scalpel handles, #4
- Scalpel blades, #22, #4 handles
- Trays, plastic
Part 1: Separating Cell Structures
- Put a raw egg in a beaker of white vinegar. Cover it with plastic wrap and label it. Leave it for at least one day, while that's happening move on to Part 2.
- After at least one day, take the egg out of the beaker of vinegar. The shell will have dissolved in the vinegar, pick it up with the slotted spoon, and rinse the vinegar off.
- Touch whats left of the outer membrane. To see that water can still go in, leave it in a beaker of 5% sodium chloride for one day.
- Rinse the egg of with water. Then, place the egg in a beaker of distilled water for one day.
- Cut open the egg cells membranes, and let the egg white slowly drip out through the slotted spoon into a 100 ml beaker. Do this step carefully so as not to pierce the egg yolk itself.
- Put the egg yolk in a different beaker, and put the egg membranes to the side.
- Using the table and steps below, test four substances with their indicator substance. Record the results and all necessary data.
- Testing for glucose: in a clean test tube, mix 2 ml of a 2% glucose substance with 2 ml of Benedict's solution. in a boiling water bath(100 degrees C of 250 ml water) heat for 2 minutes. Record the color changes and the time it takes for each change.
- Testing water: in a test tube, put 2 ml of deiodized water in a test tube and 2 ml of Benedict's solution. Put them in a boiling bath of water, 250 ml of water at 100 degrees C, heat them for 2 minutes. Observe the changes and record how long it takes for it to change.
- Testing for starch: in a test tube, put in 2 ml of well-mixed starch, and put in 2 ml Lugol's Iodine Solution. gently swirl. Record the color changes and the time it takes.
- Testing for water: in a test tube, mix 2 ml of deiodinized water and 2 ml Lugol's Iodine Solution. gently mix.record the color changes and the time it takes.
- Testing for starch: In a test tube, mix 2 ml of starch and 2 ml of Lugol's Iodine Solution. Mix it. Record the changes.
- Testing water: Place 2 ml of deionized water in a test tube. Add 0.5 ml of 10% NaOH, and gently vortex. Add 0.25 ml of 5% CuSO4. Record color change.
Lipid Indicator Test
- Testing lipids: Place a drop of fat on a piece of brown paper bag. Leave it alone for ten minutes. Hold it up to the light and record the amount of light that passes through it.
- Testing water: Place a drop of water on a paper bag. Let it sit for ten minutes. Hold it up to light and record how much you can see through it.
- Do every test from part 2, but substitute the fat, lipids and proteins with parts of the egg.
- Record the results of every test on a new table.
- Assign each table a number and compare it with the other tables.
In the first day of this lab, we were tasked with placing an egg in a beaker filled to the top with vinegar, to see the reaction that would occur between the egg and the vinegar.
On the second day that we observed the egg, the eggs shell was dissolved. To remove all remnants of the H20 from the egg, the egg was placed in sat water.
On the third day of observing the egg, we needed to rehydrate the egg, It was placed back into distilled water. At that point, we cut the egg open to remove all the parts of it to test them, which we did. We were meant to collect the data, which is below.
for this experiment, we worked with indicator tests.these tests are very important for testing for certain substances that may be harmful for living organisms.
we tested it with certain substances and also with different part of an egg.
In this experiment, a lot of things could've gone wrong, and probably did, but whatever, number one rule of STEM, don't snitch. Also, human error and adding too much of one material into a substance. but mostly, ain't no snitching in STEM.
we tested it with certain substances and also with different part of an egg.
In this experiment, a lot of things could've gone wrong, and probably did, but whatever, number one rule of STEM, don't snitch. Also, human error and adding too much of one material into a substance. but mostly, ain't no snitching in STEM.